Deletion screening at the hprt gene in V79 Chinese hamster cells using the polymerase chain reaction (PCR)
✍ Scribed by B. Köberle; S. Haupter S.; C. Röscheisen; G. Speit
- Book ID
- 113252498
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 86 KB
- Volume
- 271
- Category
- Article
- ISSN
- 0165-1161
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Spontaneous phenotypic revertants of hypoxanthine phosphoribosyl-transferase (HPRT) temperature-sensitive V79 Chinese hamster cells were selected by plating a temperature-sensitive mutant in HAT medium at 39OC. The incidence of such revertants was approximately 2 x per cell. The majority of the reve
In the present study the carcinogenic metal ions Cd [II], Co [II], Cr [VI], Ni [II], and Pb [II], as well as As[III], were examined for their ability to induce intrachromosomal homologous and nonhomologous recombination in the hprt gene of two V79 Chinese hamster cell lines, SPD8 and Sp5, respective