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Isolation and characterization of spontaneously occurring mutations at the HPRT locus in V79 Chinese hamster cells

✍ Scribed by Li-Hua Zhang; Dag Jenssen

Book ID
113193618
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
809 KB
Volume
212
Category
Article
ISSN
0027-5107

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Spontaneous phenotypic revertants of hypoxanthine phosphoribosyl-transferase (HPRT) temperature-sensitive V79 Chinese hamster cells were selected by plating a temperature-sensitive mutant in HAT medium at 39OC. The incidence of such revertants was approximately 2 x per cell. The majority of the reve