Degradation of ribonucleic acid in Candida lipolytica: Extraction of ribonucleic acid degrading enzymes
โ Scribed by Akira Imada; Anthony J. Sinskey; Steven R. Tannenbaum
- Publisher
- John Wiley and Sons
- Year
- 1972
- Tongue
- English
- Weight
- 752 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
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A simple technique for the electrophoretic extraction of RNA, under constant monitoring by bromphenol blue line, in a concentrated form from agarose-a&amide composite gels run according to A. C. Peacock and C. W. Dingman, (Biochemistry 7,668-674, (1968)) has been developed. Typically, the extraction
Suspensions of Staphylococcus aureus were held at 50 or 60" in water, 1 M sucrose, or 0.5 M sodium chloride, and analyses were made of cellular ribonucleic acid (RNA), the content of RNA-like material in the acid-soluble (metabolic) pool and the amount of RNA-like material leaked. In sucrose and sod