Influence of ionic and nonionic materials on thermally-induced ribonucleic acid degradation and leakage in Staphylococcus aureus
✍ Scribed by M. C. Allwood; A. D. Russell
- Publisher
- John Wiley and Sons
- Year
- 1970
- Tongue
- English
- Weight
- 389 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0022-3549
No coin nor oath required. For personal study only.
✦ Synopsis
Suspensions of Staphylococcus aureus were held at 50 or 60" in water, 1 M sucrose, or 0.5 M sodium chloride, and analyses were made of cellular ribonucleic acid (RNA), the content of RNA-like material in the acid-soluble (metabolic) pool and the amount of RNA-like material leaked. In sucrose and sodium chloride, as compared to water, RNA degradation was reduced at 50" and increased at 60". In comparison to water, Mg++ ions reduced RNA-like material leaked at 37 and 50", but not at 60". The results are discussed in relation to the effect of thermal damage and loss of viability of the organism.
Keyphrases
Ribonucleic acid (RNA) degradation, leakagethermally induced Degradation, Staphylococcus aureus RNAtemperature effect c] Ionic, nonionic materials effect-RNA degradation 0 UV spectrophotometry-analysis
Previous studies (1-4) have been concerned with the reasons for thermally-induced death in Staphylococcus aureus strain NCTC 3761. Several changes took place in heated suspensions of this organism: protein co-