Decarboxylation of l-tyrosine and l-dopa by immobilized cells of Papaver somniferum
✍ Scribed by Ján Stano; Pavel Nemec; Kristína Weissová; Peter Kovács; Daniela Kákoniová; Desana Lisková
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 185 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0031-9422
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The present report describes a highly sensitive method for measuring tyrosine hydroxylase based on the enzymic decarboxylation of 14Ccarboxyl labeled n-dihydroxyphenylalanine (dopa) formed from car-boxy1 labeled n-tyrosine. Radiochemical methods for the determination of tyrosine hydroxylase activity
After the isolation of an alkaloid from Papaver somniferum L. var. nigrum, its structure was determined by means of IR, UV and 1 H and 13 C-NMR spectroscopy, and MS, together with a selective O-methylation assay. The alkaloid was identified as 6,7,8,14-tetrahydro-4,5-epoxy-6-methoxy-17-methyl morphi
Direct brominatbn of L-dopa with stoichbmetric amounts of molecular bromine was found to yield 6-bromo-L-dopa as the sole product. This determination was made using liquid chromatography with electrochemical detector, mass spectroscopy, and proton NMR. 6-Bromo-L-dopa was found to be a substrate of a