## Abstract ## Background Homogeneity of cell populations is a basic requirement for gene expression analyses of the cell cycle, such as those based on microarrays. The most common approach to obtain specific populations is the use of synchronization methods that increase the number of cells repre
Cytoplasmic levels of IgE(Δ) in human myeloma cells in the different phases of the cell cycle
✍ Scribed by D. Killander; K. Nilsson; Lena Lundin; H.Å. Fabricius
- Publisher
- John Wiley and Sons
- Year
- 1977
- Tongue
- English
- Weight
- 707 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0014-2980
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Abstract Freshly explanted human myeloma cells formed colonies of monoclonal plasma cells in soft agar in the presence of medium conditioned by the adherent spleen cells of mineral oil‐primed BALB/c mice. The medium showed peak activity at a dilution of 1:4. 2‐mercaptoethanol or monothioglycerol
After testing the BrdUrd technique on experimental tumour cell lines, we applied the technique to human renal cell carcinoma in vitro. We compared the results with the data acquired after FCM analysis and 3H-thymidine treatment. In contrast to BrdUrd the 3H-thymidine uptake seemed to be limited in s