๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

The nature of cells generating human myeloma colonies in vitro

โœ Scribed by Anne W. Hamburger; Mary B. Kim; Sydney E. Salmon

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
456 KB
Volume
98
Category
Article
ISSN
0021-9541

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โœฆ Synopsis

Abstract

Freshly explanted human myeloma cells formed colonies of monoclonal plasma cells in soft agar in the presence of medium conditioned by the adherent spleen cells of mineral oilโ€primed BALB/c mice. The medium showed peak activity at a dilution of 1:4. 2โ€mercaptoethanol or monothioglycerol was necessary for colony formation. Other thiols tested were ineffective in promoting colony growth. Colonyโ€forming cells adhered to nylon wool, but not glass beads or plastic dishes. The presence of Eโ€rosetting cells was not required for myeloma colony formation. Antibody prepared against a human myeloma cell line, RPMI 8226, reduced colony formation. These studies demonstrate the usefulness of this bioassay for determining functional properties of the myeloma colonyโ€forming cell.

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