## Abstract Synchronous daughter cells of __Chlorella pyrenoidosa__ can be selected, on the basis of their unique density, from cultures growing in continuous light by centrifugation to equilibrium in aqueous Ficoll. The degree of synchrony, as judged from cell release data, is equivalent to or bet
Selection of ehrlich tumor cells in different phases of the cell cycle by ficoll gradient centrifugation
โ Scribed by A. Lepoint
- Publisher
- Springer-Verlag
- Year
- 1977
- Tongue
- English
- Weight
- 405 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0340-6075
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Using the double thymidine block technique, Ehrlich ascites tumor cells (ELD) carried i n continuous spinner culture have been synchronized. Simultaneous monitoring of :tH-thymidine incorporation, cell number and mitotic index yielded a cell cycle time of approximately 13.5 hours. This is composed o
## Abstract ## Background Homogeneity of cell populations is a basic requirement for gene expression analyses of the cell cycle, such as those based on microarrays. The most common approach to obtain specific populations is the use of synchronization methods that increase the number of cells repre