Cytochemical characteristics of clones of human bone marrow stromal fibroblasts
β Scribed by V. S. Astakhova; D. F. Gluzman; L. Yu. Poludnenko
- Book ID
- 104964256
- Publisher
- Springer US
- Year
- 1983
- Tongue
- English
- Weight
- 586 KB
- Volume
- 96
- Category
- Article
- ISSN
- 0007-4888
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## ABSTRACT Aim of the study was the establishment of a standardized in vitro culture system for studies on proliferation and differentiation of human leukemic pre β B cells. Coincubation with human stromal cells led to a significantly higher proliferation of the cytokine β sensitive leukemic pre β
## Abstract In a liquid culture of human bone marrow, the development of fibroblast colonies takes place on days 6 to 9. Twenty percent fetal calf serum is used as the stimulus for fibroblast colony growth. Human bone marrow cells are plated as 2 Γ 10~5~ cells in the culture. Normal human bone marr