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Proliferation of human leukemic pre-B cells induced by human bone marrow stromal cells and murine fibroblasts.

✍ Scribed by Christian Buske; Wolfgang Hiddemann; Bernhard Wörmann

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
426 KB
Volume
18
Category
Article
ISSN
1065-6995

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✦ Synopsis

ABSTRACT

Aim of the study was the establishment of a standardized in vitro culture system for studies on proliferation and differentiation of human leukemic pre ‐ B cells. Coincubation with human stromal cells led to a significantly higher proliferation of the cytokine ‐ sensitive leukemic pre ‐ B cell line BLIN‐1. Clones from the murine fibroblast cell line L929 provided identical results. Coincubation with the murine cells also resulted in a significantly higher numbers of viable cells in 5 of 8 patient samples with newly diagnosed B lineage ALL. The results show that in vitro bone marrow stromal cells can be substituted by murine fibroblasts and form the basis for a simpler and more reproducible assay system.

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