𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Gene expression profile of human bone marrow stromal cells determined by restriction fragment differential display analysis

✍ Scribed by Massimiliano Monticone; Yi Liu; Laura Tonachini; Maddalena Mastrogiacomo; Stefano Parodi; Rodolfo Quarto; Ranieri Cancedda; Patrizio Castagnola

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
270 KB
Volume
92
Category
Article
ISSN
0730-2312

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Using an in vitro osteogenic culture system, we carried out a restriction fragment differential display (RFDD‐PCR) to identify genes expressed by these cells in their undifferentiated stage and not expressed, or expressed at a lower level, in a closely related but distinct cell type: bone marrow stromal cells (BMSC)‐derived osteoblasts (BDO). Forty‐seven candidate regulated genes, selected by RFDD, were analyzed by RT‐PCR analysis in three cell clones and in primary cultures from seven different donors. A subset of three genes were confirmed as upregulated in BMSC relative to BDO in every primary culture and cloned population examined: βIG‐h3, IGFbp3, and LOXL2. Their differential expression was confirmed by Northern analysis and the corresponding proteins were detected by immunolocalization in BMSC. © 2004 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Analysis of activin A gene expression in
Analysis of activin A gene expression in human bone marrow stromal cells
✍ Karen E. Dolter; Julie C. Palyash; Li-En Shao; John Yu 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 253 KB 👁 2 views

Activin A, a member of the TGF-␤ superfamily, plays roles in differentiation and development, including hematopoiesis. Our previous studies indicated that the expression of activin A by human bone marrow cells and monocytes is highly regulated by inflammatory cytokines and glucocorticoids. The prese

Differential display analysis of breast
Differential display analysis of breast carcinoma cells enriched by immunomagnetic target cell selection: Gene expression profiles in bone marrow target cells
✍ Anne Hansen Ree; Olav Engebraaten; Eivind Hovig; Øystein Fodstad 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 French ⚖ 121 KB

## Abstract The red bone marrow (BM) is an important indicator organ of hematogenous micrometastatic spread of carcinomas. Characterization of biological properties specific for BM micrometastatic cells, however, is technically challenging due to the limited number of target cells usually available

Cartilage-like gene expression in differ
Cartilage-like gene expression in differentiated human stem cell spheroids: A comparison of bone marrow–derived and adipose tissue–derived stromal cells
✍ Anja Winter; Stephen Breit; Dominik Parsch; Karin Benz; Eric Steck; Hans Hauner; 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 342 KB 👁 2 views

## Abstract ## Objective To compare the chondrogenic potential of human bone marrow–derived mesenchymal stem cells (BMSC) and adipose tissue–derived stromal cells (ATSC), because the availability of an unlimited cell source replacing human chondrocytes could be strongly beneficial for cell therapy

Differential display RT-PCR analysis of
Differential display RT-PCR analysis of ECV304 endothelial-like cells infected with dengue virus type 2 reveals messenger RNA expression profiles of multiple human genes involved in known and novel roles
✍ Kingsley J.L. Liew; Vincent T.K. Chow 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 248 KB 👁 1 views

## Abstract Differential display (DD)‐RT‐PCR was employed to analyze mRNAs from ECV304 human endothelial‐like cells undergoing apoptosis following infection with the virulent New Guinea C strain of dengue virus type 2 in order to elucidate the cellular gene responses to dengue viral infection at th