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Fibroblast colonies in monolayer cultures of human bone marrow

✍ Scribed by Tadami Nagao; Mitsumoto Komatsuda; Kunihiko Yamauchi; Shigeru Arimori

Publisher: John Wiley and Sons
Year: 1981
Tongue: English
Weight: 478 KB
Volume: 108
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041080206

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

In a liquid culture of human bone marrow, the development of fibroblast colonies takes place on days 6 to 9. Twenty percent fetal calf serum is used as the stimulus for fibroblast colony growth. Human bone marrow cells are plated as 2 × 10~5~ cells in the culture. Normal human bone marrow yields 47 ± 4 fibroblasts colonies per 2 × 10~5~ cells plated. Bone marrow fibroblast cultures using agar or methylcellulose restrict colony formation. Marked colony suppression was observed in acute leukemia, and a discrete colony number was observed in hypoplastic anemia. This fibroblast culture method should be applied to a larger number of patients to determine whether it has a pathognomonic value and clinical significance.

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