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Inhibition of bone resorption by 17β-estradiol in human bone marrow cultures

✍ Scribed by U. Sarma; M. Edwards; K. Motoyoshi; A. M. Flanagan

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
499 KB
Volume
175
Category
Article
ISSN
0021-9541

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✦ Synopsis

Estrogen deficiency puts individuals at risk of developing osteoporosis because it causes increased bone resorption. However, the mechanism by which this occurs is not known. We have shown, using a recently described two-phase human bone marrow culture system, that estradiol (17b-E 2 ) added to phase I results in inhibition of bone resorption by reducing the number of osteoclasts (identified as vitronectin receptor and/or calcitonin receptor-positive cells) formed in the cultures. The addition of 17b-E 2 in phase II was without effect, indicating that it does not interfere with the bone resorptive process. 17b-E 2 down-regulated mRNA expression and protein synthesis of the membrane form of macrophage colony-stimulating factor (M-CSF). 17b-E 2 did not the alter the expression of the 4.0 kb M-CSF transcript. However, it increased protein synthesis of the proteoglycan form of M-CSF, but not the 85 kDa soluble form in the same cultures. Finally, addition of M-CSF to the cultures reversed the 17b-E 2 -induced inhibitory effect. These observations suggest that regulation of the synthesis of membrane-bound M-CSF plays a role in 17b-E 2 -induced inhibition of bone resorption.

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