Dose-response studies of the induction of hyperdiploidy and polyploidy by diethylstilbestrol and 17β-estradiol in cultured human lymphocytes using multicolor fluorescence in situ hybridization
✍ Scribed by M. Schuler; L. Hasegawa; R. Parks; M. Metzler; D. A. Eastmond
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 438 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
Diethylstilbestrol (DES) and 17b-estradiol (E 2 ) are 30 mM, whereas E 2 showed its highest induction at known inducers of aneuploidy and polyploidy in 75 mM with 7% hyperdiploid cells. To distinguish vivo and in vitro. Isolated human lymphocytes were hyperdiploidy from polyploidy, a FISH labeling treated with the stilbene estrogen DES (0.05-50 strategy to detect multiple chromosomes simultane-mM) and the steroid estrogen E 2 (0.05 -75 mM) in ously was established. Using this approach, we culture. Multicolor fluorescence in situ hybridization could show that most of the cells showing multiple (FISH) with DNA probes for the centromere and hybridization regions after treatment with both adjacent heterochromatin regions of chromosomes chemicals were most likely the result of polyploidy 1, 9, and 16 was used to detect hyperdiploidy, rather than true hyperdiploidy. These results indipolyploidy, and chromosomal breakage affecting cate that the induction of hyperdiploidy/polyploidy these chromosomes. Using this FISH technique, sig-with DES and E 2 show sublinear dose-response relanificant nonlinear increases in hyperdiploidy were tionships with likely threshold concentrations in huobserved with both compounds, whereas no induc-man lymphocytes and that FISH with multiple probes tion of chromosomal breakage affecting the peri-targeting different chromosomes can be used to esticentric heterochromatin regions of chromosomes 1, mate hyperdiploidy and polyploidy frequencies. 9, and 16 could be detected.