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Factors Affecting the Cloning Efficiency of Fibroblasts Colonies in Human Bone Marrow Cultures

โœ Scribed by R. K. Chailakhyan; N. V. Latzinik; A. G. Shamsudinov; Yu. V. Gerasimov; A. I. Kuralesova; E. N. Genkina; M. R. Chailakhyan

Book ID
110329012
Publisher
Springer
Year
2002
Tongue
English
Weight
28 KB
Volume
382
Category
Article
ISSN
0012-4966

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## Abstract In a liquid culture of human bone marrow, the development of fibroblast colonies takes place on days 6 to 9. Twenty percent fetal calf serum is used as the stimulus for fibroblast colony growth. Human bone marrow cells are plated as 2 ร— 10~5~ cells in the culture. Normal human bone marr