Clonal nuture of fibroblast colonies formed by bone marrow stromal cells in culture
β Scribed by N. V. Latsinik; A. G. Grosheva; A. N. Narovlyanskii; R. G. Pavlenko; A. Ya. Fridenshtein
- Publisher
- Springer US
- Year
- 1987
- Tongue
- English
- Weight
- 252 KB
- Volume
- 103
- Category
- Article
- ISSN
- 0007-4888
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## Abstract In a liquid culture of human bone marrow, the development of fibroblast colonies takes place on days 6 to 9. Twenty percent fetal calf serum is used as the stimulus for fibroblast colony growth. Human bone marrow cells are plated as 2 Γ 10~5~ cells in the culture. Normal human bone marr
## Abstract Colonyβforming efficiency (CFE) was used to monitor the proliferative response of alkaline phosphataseβpositive rabbit bone marrow stromal cells to acute blood loss. The CFE of animals subjected to a 1% blood loss was 0.97 compared with 0.06 (p < 0.01) in nonbled animals. Sera obtained