✦ LIBER ✦

cysB and cysE mutants of Escherichia coli K12 show increased resistance to novobiocin

✍ Scribed by Rakonjac, Jasna ;Milic, Mirjana ;Savic, Dragutin J.

Publisher: Springer
Year: 1991
Tongue: English
Weight: 506 KB
Volume: 228
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00282481

No coin nor oath required. For personal study only.

✦ Synopsis

Mutations in the cysB and cysE genes of Escherichia coli K12 cause an increase in resistance to the gyrase inhibitor novobiocin but not to coumermycin, acriflavine and rifampicin. This unusual relationship was also observed among spontaneous novobiocin resistant (Novr) mutants: 10% of Novr mutants isolated on rich (LA) plates with novobiocin could not grow on minimal plates, and among those approximately half were cysB or cysE mutants. Further analyses demonstrated that cysB and cysE negative alleles neither interfere with transport of novobiocin nor affect DNA supercoiling.

📜 SIMILAR VOLUMES

Characterisation of mutants of Escherich

Characterisation of mutants of Escherichia coli K12, selected by resistance to streptozotocin

✍ Lengeler, J. 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 586 KB

From cultures of sensitive bacteria, treated with the antibiotic streptozotocin, two classes of resistant mutants can be isolated: 1) mutants, resistant under all the conditions tested to even the highest doses of the antibiotic. These are either pleiotropic-defective, pts-mutants, or more frequentl

Genetic characterization of streptomycin

Genetic characterization of streptomycin-resistant and-dependent mutants of Escherichia coli K12

✍ Sánchez-Anzaldo, F. J. ;Bastarrachea, Fernando 📂 Article 📅 1974 🏛 Springer 🌐 English ⚖ 1003 KB

Isolation of mutants of Escherichia coli

Isolation of mutants of Escherichia coli with increased resistance to alkylating agents: Mutants deficient in thiols and mutants constitutive for the adaptive response

✍ Sedgwick, Barbara ;Robins, Peter 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 481 KB

Isolation and characterization of mutant

Isolation and characterization of mutants with a feedback resistant N-acetylglutamate synthase in Escherichia coli K 12

✍ Eckhardt, Thomas ;Leisinger, Thomas 📂 Article 📅 1975 🏛 Springer 🌐 English ⚖ 493 KB

Mutants with a feedback resistant N-acetylglutamate synthase have been isolated from a proA/B, argD, argR strain by screening for proline excretion on minimal medium with arginine. The feedback resistant character of three mutants was transduced into an argA (N-acetylglutamate synthase negative) str

Serine sensitivity of Escherichia coli K

Serine sensitivity of Escherichia coli K 12: Partial characterization of a serine resistant mutant that is extremely sensitive to 2-ketobutyrate

✍ Danchin, Antoine ;Dondon, Liliane 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 937 KB

E. coli wild type bacteria display sensitivity towards serine. A selection medium is described which allows selection of serine resistant mutants. One such mutant is described which presents pleiotropic alterations: it exhibits a thermosensitive growth pattern, alteration in the metabolism of the pp

Transposon-insertion mutants of Escheric

Transposon-insertion mutants of Escherichia coli K12 defective in a component common to galactose and ribose chemotaxis

✍ Harayama, Shigeaki ;Palva, E. Tapio ;Hazelbauer, Gerald L. 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 855 KB

From a collection of 8,000 transposon-insertion mutants of Escherichia coli K12 we identified two mutations, trg-1::Tn5 and trg-2::Tn10, that simultaneously eliminate chemotactic response to ribose and galactose, two attractants recognized by independent receptors. We show that these transposon-inse