Serine sensitivity of Escherichia coli K 12: Partial characterization of a serine resistant mutant that is extremely sensitive to 2-ketobutyrate
✍ Scribed by Danchin, Antoine ;Dondon, Liliane
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 937 KB
- Volume
- 178
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
E. coli wild type bacteria display sensitivity towards serine. A selection medium is described which allows selection of serine resistant mutants. One such mutant is described which presents pleiotropic alterations: it exhibits a thermosensitive growth pattern, alteration in the metabolism of the pppGpp and ppGpp nucleotides, cAMP intracellular level alteration, extreme sensitivity to 2-ketobutyric acid and a defect in the phosphotransferases permeation system. A conjecture explaining these apparently unrelated defects supposes that serine metabolism interferes via phosphoenol pyruvate with a cytoplasmic control of membrane activity (the mutant would be defective in the coupling between membrane and the protein responsible for its cytoplasmic control) and that 2-ketobutyrate is an effector of this activity.