Cycle-specific replication of chromosomal and F-plasmid origins
โ Scribed by Stephen Cooper; Jay D. Keasling
- Book ID
- 109322302
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 300 KB
- Volume
- 163
- Category
- Article
- ISSN
- 0378-1097
No coin nor oath required. For personal study only.
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We constructed a series of defective mini-F plasmids, which have deletion(s) in the replication origin I and/or origin II, and their derivatives, which do not produce F3 protein, by insertion of the XhoI fragment of Tn5 into the XhoI site at 41.0 F (kilobases on the coordinate map of F-plasmid). Usi
We isolated a series of Tn5-insertional mutants from the mini-F plasmid, which has a deletion in the origin II region and replicates exclusively from origin 1, and found that the mutants that had Tn5 in either the F4 or the F5 gene were defective in their replication. It is concluded that, in additi