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An essential gene for replication of the mini-F plasmid from origin I

✍ Scribed by Tanimoto, Kouichi ;Iino, Tetsuo

Publisher
Springer
Year
1984
Tongue
English
Weight
551 KB
Volume
196
Category
Article
ISSN
0026-8925

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✦ Synopsis

We constructed a series of defective mini-F plasmids, which have deletion(s) in the replication origin I and/or origin II, and their derivatives, which do not produce F3 protein, by insertion of the XhoI fragment of Tn5 into the XhoI site at 41.0 F (kilobases on the coordinate map of F-plasmid). Using these mutant mini-F plasmids, we found that F3 protein is essential for the replication of mini-F from origin I, but not from origin II.

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