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Partitioning of the F plasmid: Overproduction of an essential protein for partition inhibits plasmid maintenance

✍ Scribed by Kusukawa, Noriko ;Mori, Hirotada ;Kondo, Akihiro ;Hiraga, Sota


Publisher
Springer
Year
1987
Tongue
English
Weight
860 KB
Volume
208
Category
Article
ISSN
0026-8925

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At the XhoI site (45.08F) of plasmid mini-F a deletion of 649 bp was generated employing exonuclease Bal31. By this deletion nucleotide sequences functioning as origin II and the four 19 bp direct repeats constituting the incB region in front of the E protein gene were removed from the plasmid. Anal