Partitioning of the F plasmid: Overproduction of an essential protein for partition inhibits plasmid maintenance
β Scribed by Kusukawa, Noriko ;Mori, Hirotada ;Kondo, Akihiro ;Hiraga, Sota
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 860 KB
- Volume
- 208
- Category
- Article
- ISSN
- 0026-8925
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We constructed a series of defective mini-F plasmids, which have deletion(s) in the replication origin I and/or origin II, and their derivatives, which do not produce F3 protein, by insertion of the XhoI fragment of Tn5 into the XhoI site at 41.0 F (kilobases on the coordinate map of F-plasmid). Usi
At the XhoI site (45.08F) of plasmid mini-F a deletion of 649 bp was generated employing exonuclease Bal31. By this deletion nucleotide sequences functioning as origin II and the four 19 bp direct repeats constituting the incB region in front of the E protein gene were removed from the plasmid. Anal