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Additional genes essential for replication of the mini-F plasmid from origin I

✍ Scribed by Tanimoto, Kouichi ;Iino, Tetsuo

Publisher
Springer
Year
1985
Tongue
English
Weight
203 KB
Volume
198
Category
Article
ISSN
0026-8925

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✦ Synopsis

We isolated a series of Tn5-insertional mutants from the mini-F plasmid, which has a deletion in the origin II region and replicates exclusively from origin 1, and found that the mutants that had Tn5 in either the F4 or the F5 gene were defective in their replication. It is concluded that, in addition to the F3 gene on which we have reported previously, both the F4 and the F5 genes are essential for the replication from origin I.

πŸ“œ SIMILAR VOLUMES

An essential gene for replication of the
An essential gene for replication of the mini-F plasmid from origin I
✍ Tanimoto, Kouichi ;Iino, Tetsuo πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 551 KB

We constructed a series of defective mini-F plasmids, which have deletion(s) in the replication origin I and/or origin II, and their derivatives, which do not produce F3 protein, by insertion of the XhoI fragment of Tn5 into the XhoI site at 41.0 F (kilobases on the coordinate map of F-plasmid). Usi

The repeated sequences (incB) preceding
The repeated sequences (incB) preceding the protein E gene of plasmid mini-F are essential for replication
✍ DisquΓ©-Kochem, Claudia ;Seidel, Ulla ;Helsberg, Matthias ;Eichenlaub, Rudolf πŸ“‚ Article πŸ“… 1986 πŸ› Springer 🌐 English βš– 515 KB

At the XhoI site (45.08F) of plasmid mini-F a deletion of 649 bp was generated employing exonuclease Bal31. By this deletion nucleotide sequences functioning as origin II and the four 19 bp direct repeats constituting the incB region in front of the E protein gene were removed from the plasmid. Anal