✦ LIBER ✦
DNA sequence of an amber replication mutant indicates that a 29 kd protein is the product of the F plasmid replication gene
✍ Scribed by Maki, Satoko ;Miki, Takeyoshi ;Horiuchi, Tadao
- Publisher
- Springer
- Year
- 1984
- Tongue
- English
- Weight
- 197 KB
- Volume
- 194
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
DNA sequencing'shows that the mutational alteration resulting from an amber-suppressible replicationdefective mutation of F plasmid is a single base pair change from C:G to T:A which yields an amber codon in the coding frame for a 29 kd polypeptide located in the minimal replication region. We thus identified the gene indispensable for F DNA replication as the coding frame which encodes a 29 kd polypeptide. We will designate this gene repA.