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Separation of the minimal replication region of the F plasmid into a replication origin segment and a trans-acting segment

โœ Scribed by Tolun, Ashhan ;Helinski, Donald R.


Publisher
Springer
Year
1982
Tongue
English
Weight
700 KB
Volume
186
Category
Article
ISSN
0026-8925

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Yeast DNA pools were prepared by ligating partial Sau3A genomic digests from strains carrying various MAL genes into the BamHI site of the yeast-Escherichia coli shuttle vector YRp7. They were used to transform recipient yeast strains that could not utilize maltose since they lacked a classical MAL