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Replication and incompatibility properties of segments of the origin region of replication of the broad host range plasmid RK2

✍ Scribed by Thomas, Christopher M. ;Stalker, David M. ;Helinski, Donald R.


Publisher
Springer
Year
1981
Tongue
English
Weight
873 KB
Volume
181
Category
Article
ISSN
0026-8925

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πŸ“œ SIMILAR VOLUMES


Nucleotide sequence of the region of the
✍ Stalker, David M. ;Thomas, Christopher M. ;Helinski, Donald R. πŸ“‚ Article πŸ“… 1981 πŸ› Springer 🌐 English βš– 529 KB

A DNA sequence consisting of 617 base pairs (bp) from the region of the origin of replication of the broad-host range plasmid RK2 has been determined. Included within this sequence is a 393 bp HpaII restriction fragment that provides a functional origin or replication when other essential RK2 specif

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A 500 bp DNA fragment located in the vicinity of the origin of replication of plasmid RSF1010 was cloned into the plasmid vector pBR322 and shown to exhibit incompatibility against parental RSF1010. The rightmost region of this fragment contains three perfect 20 bp direct repeats and a fourth half-r

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Transcription at various points in the trf A region of broad host range plasmid RK2 has been analysed by measuring expression of the galK gene inserted at EcoRI sites introduced previously by TB1723 transposition mutagenesis. Rightward transcription (anti-clockwise on RK2) probably from a single pro

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✍ Smith, Christopher A. ;Thomas, Christopher M. πŸ“‚ Article πŸ“… 1983 πŸ› Springer 🌐 English βš– 980 KB

Figurski et al. (1982) have reported that certain loci on the broad host range plasmid RK2 (kil functions) can be cloned only in the presence of other trans-acting segments of the plasmid genome (kor functions). They have suggested that the presence of these functions may in part account for the str