✦ LIBER ✦

Crystallization and preliminary X-ray diffraction analysis of staphylococcal enterotoxin type C

✍ Scribed by Dr. Gregory A. Bohach; Young-In Chi; Cynthia V. Stauffacher

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 577 KB
Volume: 13
Category: Article
ISSN: 0887-3585
DOI: 10.1002/prot.340130208

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✦ Synopsis

Abstract

The Type C staphylococcal enterotoxin produced by Staphylococcus aureus strain FRI‐909 has been crystallized using a combination of two precipitants, ammonium sulfate and polyethylene glycol 400, with the addition of small amounts of detergent. Two related crystal forms have been obtained, one triclinic, and one tetragonal, both with one toxin molecule per asymmetric unit. These crystals are stable for at least 75 hr in the X‐ray beam and diffract to at least 2.2 and 2.6 Å, respectively. The triclinic crystals have unit cell parameters a = 38.5 Å, b = 43.7 Å, c = 36.9 Å, and interaxial angles α = 99.9°, β = 95.8°, and γ = 98.5°. The tetragonal crystals are of space group __P__4~1~22 with unit cell parameters a = 43.4 Å and c = 278.0 Å. © 1992 Wiley‐Liss, Inc.

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