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Crystallization and preliminary X-ray diffraction analysis of importin-α complexed with NLS peptidomimetics

✍ Scribed by Marcos R.M. Fontes; Trazel Teh; Ryan D. Riell; Seung Bum Park; Robert F. Standaert; Bostjan Kobe

Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
200 KB
Volume
1750
Category
Article
ISSN
1570-9639

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✦ Synopsis

Importin-alpha is the nuclear import receptor that recognizes cargo proteins with nuclear localization sequences (NLSs). The study of NLS peptidomimetics can provide a better understanding of the requirements for the molecular recognition of cargo proteins by importin-alpha, and potentially engender a large number of applications in medicine. Importin-alpha was crystallized with a set of six NLS peptidomimetics, and X-ray diffraction data were collected in the range 2.1-2.5 A resolution. Preliminary electron density calculations show that the ligands are present in the crystals.

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