✦ LIBER ✦

Crystallization and preliminary X-ray diffraction analysis of an acidic phospholipase A2 complexed with p-bromophenacyl bromide and α-tocopherol inhibitors at 1.9- and 1.45-Å resolution

✍ Scribed by Agnes A.S. Takeda; Juliana I. dos Santos; Silvana Marcussi; Lucas B. Silveira; Andreimar M. Soares; Marcos R.M. Fontes

Publisher: Elsevier Science
Year: 2004
Tongue: English
Weight: 110 KB
Volume: 1699
Category: Article
ISSN: 1570-9639
DOI: 10.1016/j.bbapap.2004.02.005

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✦ Synopsis

An acidic phospholipase A 2 (PLA 2 ) isolated from Bothrops jararacussu snake venom was crystallized with two inhibitors: a-tocopherol (vitamin E) and p-bromophenacyl bromide (BPB). The crystals diffracted at 1.45-and 1.85-A ˚resolution, respectively, for the complexes with a-tocopherol and p-bromophenacyl bromide. The crystals are not isomorphous with those of the native protein, suggesting the inhibitors binding was successful and changes in the quaternary structure may have occurred.