✦ LIBER ✦

Coupling matrix-assisted laser desorption/ionization to liquid separations

✍ Scribed by Kermit K. Murray

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 339 KB
Volume: 16
Category: Article
ISSN: 0277-7037
DOI: 10.1002/(sici)1098-2787(1997)16:5<283::aid-mas3>3.0.co;2-d

No coin nor oath required. For personal study only.

✦ Synopsis

This review discusses the off-line and on-line coupling of matrixspray ionization (ESI) (Fenn et al., 1990) have revolutionassisted laser desorption/ionization (MALDI) mass spectrometry to ized the mass spectrometric analysis of large molecules.

liquid chromatography (LC) and capillary electrophoresis (CE).

Mass spectrometry of biomolecules with molecular

Off-line methods involve collecting fractions or depositing a sample weights up to several hundred kilodaltons (kDa) (Chait & on a prepared target prior to MALDI analysis. Because LC fraction Kent, 1992;Senko & McLafferty, 1994; Siuzdak, 1996) collection is straightforward, off-line MALDI LC-MS is relatively and synthetic polymers with resolved oligomers up to seveasy to implement and widely used. Collecting CE fractions poses eral tens of kilodaltons (Cottrell et al., 1995; O'Connor & a greater challenge due to the small sample volume and the high McLafferty, 1995; Montaudo, 1996) is now commonplace.

voltage electrical connection that must be maintained during separation. Methods for off-line MALDI CE-MS employing a porous

📜 SIMILAR VOLUMES

Ionization Modelling of Matrix Molecules

Ionization Modelling of Matrix Molecules in Ultraviolet Matrix-assisted Laser Desorption/Ionization

✍ D. A. Allwood; P. E. Dyer; R. W. Dreyfus 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 498 KB 👁 2 views

A scheme of matrix ionization in UV matrix-assisted laser desorptiodionization is presented. Excitation to an upper lying electronic singlet state by the absorption of two photons and subsequent thermionic emission by vibronic coupling is proposed as the primary route for the formation of unprotonat

Infrared matrix-assisted laser desorptio

Infrared matrix-assisted laser desorption/ionization using a frozen alcohol matrix

✍ Sheffer, Jay D.; Murray, Kermit K. 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 55 KB 👁 2 views

Figure 1. IR MALDI mass spectrum of bovine insulin in a

Matrix-assisted Laser Desorption/Ionizat

Matrix-assisted Laser Desorption/Ionization by Two Collinear Subthreshold Laser Pulses

✍ Xiaodong Tang; Mehrnoosh Sadeghi; Zohra Olumee; Akos Vertes 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 151 KB 👁 2 views

Two nitrogen laser pulses of subthreshold irradiance were used to achieve matrix-assisted laser desorption/ionization in a collinear configuration. Optimum laser pulse delay times yielding maximum guest ion intensities were determined for four different matrices between 4.2 ns for sinapinic acid (SA

Compact Tunable Cr:LiSAF Laser for Infra

Compact Tunable Cr:LiSAF Laser for Infrared Matrix-assisted Laser Desorption/Ionization

✍ Mehrnoosh Sadeghi; Zohra Olumee; Xiaodong Tang; Akos Vertes; Zhi-Xing Jang; Angu 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 105 KB 👁 2 views

A tunable Cr:LiSAF laser-pumped optical parametric oscillator was used for mid-infrared matrix-assisted laser desorption/ionization (MALDI) mass spectrometry experiments. The mass spectra of substance P, bovine insulin and pd(T) 10 in the 2.88-2.96 µm range showed excellent single shot signal qualit

Matrix-assisted laser desorption/ionizat

Matrix-assisted laser desorption/ionization for short tandem repeat loci

✍ N. I. Taranenko; V. V. Golovlev; S. L. Allman; N. V. Taranenko; C. H. Chen; J. H 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 155 KB 👁 2 views

Matrix-assisted laser desorption/ionization was used for the detection of four base short tandem repeats (STR) for clinical samples using a time-of-flight mass spectrometer. Since STR plays an important role in genetic disease and human identification, this work indicates that laser desorption mass

Chemical reactivity in matrix-assisted l

Chemical reactivity in matrix-assisted laser desorption/ionization mass spectrometry

✍ C. Enjalbal; B. Sauvagnat; F. Lamaty; R. Lazaro; J. Martinez; P. Mouchet; F. Rou 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 139 KB 👁 2 views

During the control of a multistep organic synthesis on a soluble polymer (PEG) by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, a chemical reactivity was encountered when the matrix was acidic, for the samples where the amino moiety of the anchored compounds was protected as