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Coupling dilute protein to CNBr-agarose

โœ Scribed by YAGER, T; BARRETT, D

Book ID
123101640
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
438 KB
Volume
802
Category
Article
ISSN
0304-4165

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## Coupling of alkylamines to cyanogen bromide-activated agarose results in the formation of N-substituted isoureas, which are good nucleophiles. The nucleophilic attack of these isoureas on the active chromogenic esterp-nitrophenyl acetate causes the release of an equivalent amount ofp-nitropheno