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Covalent binding of molecules to CNBr-activated agarose: Parameters relevant to the activation and coupling reactions

โœ Scribed by Stage, David E.; Mannik, Mart

Book ID
122797570
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
815 KB
Volume
343
Category
Article
ISSN
0304-4165

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Nucleophilicity of isourea linkages in substituted agaroses: A direct method for the determination of alkylamino groups coupled to CNBr-activated agarose
โœ Moshe M. Werber ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 363 KB

## Coupling of alkylamines to cyanogen bromide-activated agarose results in the formation of N-substituted isoureas, which are good nucleophiles. The nucleophilic attack of these isoureas on the active chromogenic esterp-nitrophenyl acetate causes the release of an equivalent amount ofp-nitropheno