✦ LIBER ✦
Nucleophilicity of isourea linkages in substituted agaroses: A direct method for the determination of alkylamino groups coupled to CNBr-activated agarose
✍ Scribed by Moshe M. Werber
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 363 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Coupling
of alkylamines to cyanogen bromide-activated agarose results in the formation of N-substituted isoureas, which are good nucleophiles. The nucleophilic attack of these isoureas on the active chromogenic esterp-nitrophenyl acetate causes the release of an equivalent amount ofp-nitrophenolate. This constitutes the basis for a direct determination of alkylamino groups in substituted agaroses.