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Binding of proteins to CNBr-activated sepharose 4B

✍ Scribed by Gary S. David; Tom H. Chino; Ralph A. Reisfeld

Book ID
115904596
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
275 KB
Volume
43
Category
Article
ISSN
0014-5793

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Binding of proteins, including pp60src,
Binding of proteins, including pp60src, to activated CH-Sepharose 4B
✍ Nadine Pavloff; Jean-Michel Biquard; Marcel Mariller; Gian Carlo Rabotti; Nicole πŸ“‚ Article πŸ“… 1987 πŸ› Springer 🌐 English βš– 657 KB

Activated CH-Sepharose 4B and protein A Sepharose CL-4B can bind, selectively and non-specifically, polypeptides from chick embryo cells. The major polypeptides bound have apparent molecular masses of 57-60 kDa and 47-49 kDa and cannot be eluted by extensive washing with buffers containing detergent