Deactivation of α-chymotrypsin and α-chymotrypsin-CNBr-sepharose 4B conjugates in aliphatic alcohols
✍ Scribed by Douglas S. Clark; James E. Bailey
- Book ID
- 113264696
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 565 KB
- Volume
- 788
- Category
- Article
- ISSN
- 0167-4838
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Two different immobilized chymotrypsin derivatives were used to synthesize kyotorphin, using Nbenzoyl-L-tyrosine ethyl ester and L-arginine ethyl ester as substrates, in water-DMF media. The first was adsorbed onto Celite particles and the second was multipoint covalently attached into polyacrylamid
## Abstract Enzymes are attractive catalysts for the production of optically active compounds in organic solvents. However, their often low catalytic activity in such applications hampers their practical use. To overcome this, we investigated the effectiveness of the covalent modification of α‐chym