Binding of proteins, including pp60src, to activated CH-Sepharose 4B

✍ Scribed by Nadine Pavloff; Jean-Michel Biquard; Marcel Mariller; Gian Carlo Rabotti; Nicole Fossar; Françoise Gay; Marianne Semmel

• Book ID: 104743880
• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 657 KB
• Volume: 12
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00368881

✦ Synopsis

Activated CH-Sepharose 4B and protein A Sepharose CL-4B can bind, selectively and non-specifically, polypeptides from chick embryo cells. The major polypeptides bound have apparent molecular masses of 57-60 kDa and 47-49 kDa and cannot be eluted by extensive washing with buffers containing detergents. One of the 57-60 kDa polypeptides was identified by immunoblotting as the transforming protein of Rous Sarcoma Virus (RSV), pp60src. This polypeptide could be removed from the solid phase immunoabsorbent with 60% dimethylsulfoxide, but not with 2% SDS, 5% beta-mercaptoethanol, 1 M NaCl or 0.1% Tween 20.