Counter-current chromatography of lipoproteins with a polymer phase system using the cross-axis synchronous coil planet centrifuge
โ Scribed by Yoichi Shibusawa; Yoichiro Ito; Katsunori Ikewaki; Daniel J. Rader; H.Bryan Brewer Jr.
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 417 KB
- Volume
- 596
- Category
- Article
- ISSN
- 1873-3778
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๐ SIMILAR VOLUMES
A novel design of the cross-axis synchronous flow-through coil planet centrifuge is introduced. The apparatus holds a pair of large coil holders symmetrically, one on each side of the rotary frame, at a lateral position 12.5 cm from the center of the holder shaft held 10 cm from the centrifuge axis.
Performance of the apparatus was evaluated in terms of stationary phase retention, partition efficiency and sample loading capacity. Preliminary studies with short coils revealed high retention of the stationary phase under a proper combination of the head-tail elution and planetary motion. Preparat
A series of experiments was conducted to evaluate the partition efficiency of the cross-axis synchronous flow-through coil planet centrifuge. The preliminary studies indicated that short coils (5 m) mounted on 15-and 25-cm diameter holders in either a central or a lateral location produce a high par