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Construction and characterization of new cloning vehicle. II. A multipurpose cloning system

✍ Scribed by Bolivar, Francisco; Rodriguez, Raymond L.; Greene, Patricia J.; Betlach, Mary C.; Heyneker, Herbert L.; Boyer, Herbert W.; Crosa, Jorge H.; Falkow, Stanley

Book ID
120368555
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
930 KB
Volume
2
Category
Article
ISSN
0378-1119

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pUR2, a certified B2(EK2) vector, allows easy isolation of variants containing cloned EcoRI-fragments. Bacteria harboring plasmids without inserts make blue colonies on indicator-plates, whilst those harboring recombinant plasmids make white colonies.