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Construction of a new host-vector system inArthrobactersp. and cloning of the lipase gene

✍ Scribed by M. Morikawa, H. Daido, S. Pongpobpibool…

Book ID
120768195
Publisher
Springer
Year
1994
Tongue
English
Weight
578 KB
Volume
42
Category
Article
ISSN
1432-0614

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The 6.5 kb HindIII DNA fragment of the Lactococcus lactis subsp. cremoris H2 plasmid pDI21 was cloned into Escherichia coli POP13 with lambda NM1149, and also directly into Lactococcus lactis subsp. lactis 4125 using a newly-constructed broad host-range vector pFX1. Proteinase was expressed in both