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Construction and characterization of new host-vector systems for the enforcement-cloning method

✍ Scribed by Tamotsu, Hashimoto-Gotoh; Atsushi, Tsujimura; Kin-ya, Kuriyama; Shinji, Matsuda

Book ID
120434569
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
638 KB
Volume
137
Category
Article
ISSN
0378-1119

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In vitro recombination techniques.were used to construct a new cloning vehicle, pBR322. This plasmid, derived from pBR313, is a relaxed replicating plasmid, does not produce and is sensitive to colicin El, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc). The ant