𝔖 Bobbio Scriptorium
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Construction and properties of a new cloning vehicle, allowing direct sereening for recombinant plasmids

✍ Scribed by Rüther, Ulrich


Publisher
Springer
Year
1980
Tongue
English
Weight
221 KB
Volume
178
Category
Article
ISSN
0026-8925

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✦ Synopsis


pUR2, a certified B2(EK2) vector, allows easy isolation of variants containing cloned EcoRI-fragments. Bacteria harboring plasmids without inserts make blue colonies on indicator-plates, whilst those harboring recombinant plasmids make white colonies.


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✍ Kuhlemeier, C. J. ;Borrias, W. E. ;van den Hondel, C. A. M. J. J. ;van Arkel, G. 📂 Article 📅 1981 🏛 Springer 🌐 English ⚖ 654 KB

Two plasmids were constructed consisting of the E. coli vector pACYC184 and the cyanobacterial plasmid pUC1. These recombinants, designated pUC104 and pUC105, can be transformed to E. coli K12 as well as to the cyanobacterium Anacystis nidulans R2 and in both hosts they express their antibiotic mark