✦ LIBER ✦

Concentration-step amperometric sensor of l-ascorbic acid using cucumber juice

✍ Scribed by Shunichi Uchiyama; Yoshihiro Umetsu

Book ID: 102623584
Publisher: Elsevier Science
Year: 1991
Tongue: English
Weight: 379 KB
Volume: 255
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(91)85086-8

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✦ Synopsis

L-Ascortx acid was determmed by concentration-step amperometry uamg a thm layer of carbon felt unpregnated with cucumber ~mce as an enzyme solutlon of ascorbate oxldase The ddute fruit ~mcc was added on top of the carbon felt and the decreased current peak caused by the enzymatic reaction was measured The cucumber Jmce was prepared by filtratton and cent&gal separation The peak current was proportional to the concentration of L-ascorbs acid m the concentration range 2 5 X 10e4-1 6 X 10m3 M The results obtamed were 111 fairly good agreement with those obtamed by hquld chromatography

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