𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Concentration-step amperometric sensor for uric acid using uricase

✍ Scribed by Shunichi Uchiyama; Shuichi Suzuki; Tomohiro Sato

Book ID
102181363
Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
335 KB
Volume
2
Category
Article
ISSN
1040-0397

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✦ Synopsis

A novel amperometric method for biochemical substrates consuming oxygen in their enzyme reactions is proposed. This method is based on the measurement of the current peak observed when the sample solution is dropped on the thin membrane dipped in the enzyme solution, which is placed on the oxygen gas-selective permeable membrane. I n order to illustrate the present concept, uric acid was chosen as a measurable species and the response characteristics of the sensor were investigated. The peak current obtained by sample addition was proportional to the concentration of uric acid in the dropped solution, and the method was applied t o measure uric acid in human urine. The results obtained were in fairly good agreement with those obtained using a conventional spectrophotometric method.

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