The adaptation of an isocratic reversed-phase ion-pairing high performance liquid chromatographic method in combination with derivatization of dehydroascorbic acid by l&phenylenediamine prior to injection for simultaneous determination of ascorbic acid and dehydroascorbic acid in plant materials is
Determination of ascorbic acid and dehydroascorbic acid in juices by high-performance liquid chromatography with electrochemical detection using l-cysteine as precolumn reluctant
โ Scribed by Hiroshi Iwase; Ichiro Ono
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 484 KB
- Volume
- 654
- Category
- Article
- ISSN
- 1873-3778
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A postcolumn reactor which reduces dehydroascorbic acid to ascorbic acid for use in high-performance liquid chromatography is described. This reactor permits the direct determination of both ascorbic and dehydroascorbic acids after their chromatographic separation. 1993 Academic Press, Inc.
The concentrations of the hydrophilic antioxidants ascorbic acid and dehydroascorbic acid in plasma for some time have been considered possible biomarkers of oxidative stress. However, several problems are associated with the accurate measurement of these two compounds. We have developed and validat
Determination of dehydroascorbic acid in biological samples most commonly involves indirect measurement. The concentration is calculated by subtraction of the measured ascorbic acid concentration from that of total ascorbic acid analyzed after reduction of the dehydroascorbic acid present; a methodo