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Comparison of the xylose reductase-xylitol dehydrogenase and the xylose isomerase pathways for xylose fermentation by recombinantSaccharomyces cerevisiae

✍ Scribed by Kaisa Karhumaa; Rosa Garcia Sanchez; Bärbel Hahn-Hägerdal; Marie-F Gorwa-Grauslund


Book ID
118039964
Publisher
BioMed Central
Year
2007
Tongue
English
Weight
683 KB
Volume
6
Category
Article
ISSN
1475-2859

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To enhance metabolite transfer in the two initial sequential steps of xylose metabolism in yeast, two structural genes of Pichia stipitis, XYL } and XYL " encoding xylose reductase (XR) and xylitol dehydrogenase (XDH), respectively, were fused in frame. Four chimeric genes were constructed, encoding