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Analysis and prediction of the physiological effects of altered coenzyme specificity in xylose reductase and xylitol dehydrogenase during xylose fermentation by Saccharomyces cerevisiae

✍ Scribed by Stefan Krahulec; Mario Klimacek; Bernd Nidetzky


Book ID
118221768
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
791 KB
Volume
158
Category
Article
ISSN
0168-1656

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To enhance metabolite transfer in the two initial sequential steps of xylose metabolism in yeast, two structural genes of Pichia stipitis, XYL } and XYL " encoding xylose reductase (XR) and xylitol dehydrogenase (XDH), respectively, were fused in frame. Four chimeric genes were constructed, encoding