Expression of Bifunctional Enzymes with
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Mikael Anderlund; Peter RΓ₯dstrΓΆm; BΓ€rbel Hahn-HΓ€gerdal
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Article
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2001
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Elsevier Science
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English
β 295 KB
To enhance metabolite transfer in the two initial sequential steps of xylose metabolism in yeast, two structural genes of Pichia stipitis, XYL } and XYL " encoding xylose reductase (XR) and xylitol dehydrogenase (XDH), respectively, were fused in frame. Four chimeric genes were constructed, encoding