The effect of the inoculum age and initial xylose concentration on the activities of xylose reductase (XR) and xylitol dehydrogenase (XD) of Candida guilliemondii FTI 20037 were investigated. Under the experimental conditions used, the XR and XD activities were, respectively, NADPH and NAD or NADP d
High activity of xylose reductase and xylitol dehydrogenase improves xylose fermentation by recombinantSaccharomyces cerevisiae
✍ Scribed by Kaisa Karhumaa; Romain Fromanger; Bärbel Hahn-Hägerdal; Marie-F. Gorwa-Grauslund
- Book ID
- 105950662
- Publisher
- Springer
- Year
- 2007
- Tongue
- English
- Weight
- 218 KB
- Volume
- 73
- Category
- Article
- ISSN
- 1432-0614
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
To enhance metabolite transfer in the two initial sequential steps of xylose metabolism in yeast, two structural genes of Pichia stipitis, XYL } and XYL " encoding xylose reductase (XR) and xylitol dehydrogenase (XDH), respectively, were fused in frame. Four chimeric genes were constructed, encoding
Recombinant Saccharomyces cerevisiae TMB3001, harboring the Pichia stipitis genes XYL1 and XYL2 (xylose reductase and xylitol dehydrogenase, respectively) and the endogenous XKS1(xylulokinase), can convert xylose to ethanol. About 30% of the consumed xylose, however, is excreted as xylitol. Enhanced