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Comparison of extraction methods and assay procedures for the determination of the apparent tannin content of common beans

✍ Scribed by Andrés Carmona; Dinah S Seidl; W G Jaffé


Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
615 KB
Volume
56
Category
Article
ISSN
0022-5142

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A new colorimetric method of tannase (tannin acyl hydrolase, EC 3.1.1.20) assay has been developed using its specific substrate tannic acid. It is based on the changes in optical density of substrate tannic acid after enzymatic reaction at 530 nm. The residual tannic acid was measured by a modified