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Colorimetric Assay Method for Determination of the Tannin Acyl Hydrolase (EC 3.1.1.20) Activity

✍ Scribed by K.C. Mondal; D. Banerjee; M. Jana; B.R. Pati

Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
49 KB
Volume
295
Category
Article
ISSN
0003-2697

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✦ Synopsis

A new colorimetric method of tannase (tannin acyl hydrolase, EC 3.1.1.20) assay has been developed using its specific substrate tannic acid. It is based on the changes in optical density of substrate tannic acid after enzymatic reaction at 530 nm. The residual tannic acid was measured by a modified BSA precipitation method. This assay is very simple, reproducible, and very convenient, and with it tannase activity can be measured in relation to the growth of the organism.

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