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Comparison of axial and radial flow chromatography on protein separation speed and resolution

โœ Scribed by Yun-Ha Kim; Eun Kyu Lee

Book ID
110642281
Publisher
Springer US
Year
1996
Tongue
English
Weight
849 KB
Volume
13
Category
Article
ISSN
0256-1115

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An axial column (3 ร‚ 2.6 cm) and a radial flow column (3.5 ร‚ 5 cm) packed with DEAE Sepharose Fast Flow media was evaluated for the separation of human prothrombin from Nitschmann fraction III. Under radial flow conditions, a sample flow rate up to 14 mL/min (approximately 18 bed vols/h) was achieve