Chromatography of human prothrombin from
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Tao Sun; Ge Chen; Yiping Liu; Fengrong Bu; Meijuan Wen
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Article
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2000
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John Wiley and Sons
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English
โ 68 KB
An axial column (3 ร 2.6 cm) and a radial flow column (3.5 ร 5 cm) packed with DEAE Sepharose Fast Flow media was evaluated for the separation of human prothrombin from Nitschmann fraction III. Under radial flow conditions, a sample flow rate up to 14 mL/min (approximately 18 bed vols/h) was achieve