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Chromatography of human prothrombin from Nitschmann fraction III on Q Sepharose Fast Flow using axial and radial flow column

โœ Scribed by Sun Tao; Chen Ge; Liu Yipin; Bu Fenfrong; Wen Meijuan


Book ID
106147597
Publisher
Springer
Year
2000
Tongue
English
Weight
104 KB
Volume
23
Category
Article
ISSN
1615-7605

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Chromatography of human prothrombin from
โœ Tao Sun; Ge Chen; Yiping Liu; Fengrong Bu; Meijuan Wen ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 68 KB

An axial column (3 ร‚ 2.6 cm) and a radial flow column (3.5 ร‚ 5 cm) packed with DEAE Sepharose Fast Flow media was evaluated for the separation of human prothrombin from Nitschmann fraction III. Under radial flow conditions, a sample flow rate up to 14 mL/min (approximately 18 bed vols/h) was achieve